Large Gram's Stain Kit

The Gram’s Stain Kit from Innovating Science provides the reagents needed to perform the complete Gram staining procedure on bacterial cells. The kit includes dilute Lugol’s iodine, crystal violet stain, safranin O (1% aqueous), and ethanol. An instruction manual and safety data sheet are included. Gram staining is a foundational technique in microbiology that differentiates bacteria into Gram-positive and Gram-negative groups based on cell wall structure. The multistep procedure involves applying crystal violet as the primary stain, fixing it with Lugol’s iodine mordant, decolorizing with ethanol, and counterstaining with safranin. Gram-positive bacteria retain the crystal violet-iodine complex and appear purple, while Gram-negative bacteria lose the primary stain and take up the red safranin counterstain. This kit is designed for educational and laboratory use, providing enough reagents for multiple staining sessions in biology and microbiology coursework.